Molecular cloning, characterization, and expression analysis of Megalobrama amblycephala melanocortin receptor 3 during fasting

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 团头鲂黑素皮质素3型受体基因克隆及表达分析 DOI: 作者: 作者单位: 1. 南京农业大学无锡渔业学院, 江苏 无锡 214081;2. 中国水产科学研究院淡水渔业研究中心, 农业农村部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081 作者简介: 廖盛臣(1992-),男,硕士研究生,主要从事水产病害学研究.E-mail:602468594@qq.com 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目（31572662）；现代农业产业技术体系建设专项（CARS-45）. Molecular cloning, characterization, and expression analysis of Megalobrama amblycephala melanocortin receptor 3 during fasting Author: Affiliation: 1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural affairs;Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:黑素皮质素3型受体（MC3R）系统与动物摄食行为以及能量调控密切相关。为丰富鱼类MC3R相关基础研究，探索鱼类摄食行为与能量调控机制，本研究克隆了团头鲂（基因，采用分子生物信息学和相对荧光定量PCR方法分别对氨基酸序列保守结构域、组织表达分布和禁食条件下的表达变化等开展分析研究。结果显示，团头鲂mc3r mRNA在下丘脑、垂体、肝脏和卵巢有相对较高的表达。禁食试验结果显示，下丘脑和垂体中的转录变化与周期性摄食信号相关，其中下丘脑 mRNA在禁食72 h和禁食72 h后恢复投喂6 h表达量显著升高（<0.05）。此外，对血液皮质醇和血糖的监测结果显示，两者均在禁食14 d有显著变化，其中皮质醇水平显著高于对照组（<0.05）。综合本研究结果表明，MC3R在鲤科鱼类中具有高度相似的氨基酸序列和结构域；在组织中的转录表达变化与食物摄取有明显相关性，对调控鱼体摄食行为和能量代谢具有重要作用。 Abstract:The melanocortin system consists of melanocortin peptides derived from the proopiomelanocortin gene, five melanocortin receptors, two endogenous antagonists, and two ancillary proteins. It plays a role in many important physiological functions of terrestrial animals. Studies on rodents have affirmed the role of melanocortin receptor 3 () in feeding behavior and energy regulation. However, the function of in fish, including basic information, is not well understood. Thus, to determine the initial value of fish and assess the role of MC3R in fish feeding behavior and energy regulation, the full-length cDNA of was cloned. The nucleotide sequence and deduced amino acid sequence were analyzed using bioinformatics methods. Blood glucose and cortisol levels were detected, and real-time quantitative PCR was employed to characterize the tissue expression profile and expression during fasting and refeeding. The results showed that cDNA encodes a protein of 327 amino acids, which shares a high amino acid sequence identity with that of other animals and is characteristic with the typical seven transmembrane domain. Phylogenetic analyses showed that the cluster was within the MC3R clade of the Cyprinidae MC3Rs. The tissue expression analysis revealed that was highly expressed in the hypothalamus, pituitary, liver, and ovary, whereas its expression was much lower in the other detected tissues. During fasting and refeeding, mRNA expression in the hypothalamus and pituitary showed a periodic variation with higher expression near the first feeding time every day both in the experimental and control groups. Furthermore, the expression of mRNA was significantly increased and was higher than that in the control group after 72 h and 14 d fasting in the hypothalamus ( < 0.05), whereas similar results occurred in the pituitary at 72 h fasting and 6 h after refeeding ( < 0.05). In addition, an obvious change in blood glucose and cortisol was only observed with long term fasting ( is not only highly conservative in genetic information, but also in its tissue expression profile and physiological function during the evolutionary process, and may play an important role in feeding behavior and energy regulation. 参考文献 相似文献 引证文献