Abstract
P53 And DNA Damage-Regulated Gene 1 (PDRG1) is a novel gene which plays an important role in chaperone-mediated protein folding. In the present study, the full-length complementary DNA (cDNA) sequence of the PDRG1 gene from Penaeus monodon (PmPDRG1) was cloned by the rapid amplification of cDNA ends (RACE) method. The cDNA of PmPDRG1 spans 1,613 bp, interrupted by only one short intron, and encodes a protein of 136 amino acids with calculated molecular weight of 15.49 kDa. The temporal expression profile of PmPDRG1 in different tissues and in different developmental stages of the ovary was investigated by real-time quantitative PCR (RT-qPCR). An RNA interference (RNAi) experiment was performed to study the relationship between P. monodon p53 (Pmp53) and PmPDRG1, and the results showed that the relative expression level of PmPDRG1 mRNA was notably up-regulated from 12 h to 96 h after Pmp53 was silenced both in ovary and hepatopancreas. To further explore the role of PmPDRG1 in ovarian development, dopamine (DA) and 5-hydroxytryptamine (5-HT)-injected shrimps were analyzed by RT-qPCR, indicating that PmPDRG1 may be involved in the regulation of ovarian development of P. monodon.
Highlights
P53 And DNA Damage-Regulated Gene 1 (PDRG1, namely was first identified in 2003 by Luo et al (2003)
The full-length complementary DNA (cDNA) sequence of PmPDRG1 was obtained by rapid amplification of cDNA ends (RACE)-PCR, and the complete nucleotide sequence and the deduced amino acid sequence are shown in Figure 1a (GenBank: KX156929)
PmPDRG1 is encoded in the nuclear genome, and has only one intron in the open reading frame (ORF) (Figure 1b)
Summary
P53 And DNA Damage-Regulated Gene 1 (PDRG1, namely was first identified in 2003 by Luo et al (2003). PDRG1 is usually strongly over-expressed in multiple human malignancies (Jiang et al, 2011; Wang et al, 2015a). PDRG1 protein was identified as a subunit of the R2TP/prefoldin-like complex, which is involved in the assembly of the RNA polymerase II complex (Pol II) in the cytoplasm of eukaryotic cells (Sardiu et al, 2008; Boulon et al, 2012; Mita et al, 2013). The tumor suppressor protein p53 can down-regulate the expression of PDRG1 mRNA, while ultraviolet (UV) radiation has the opposite effect (Luo et al, 2003).
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