Molecular Cloning and Tissue-specific Expression of Intestinal-type Fatty Acid Binding Protein in Porcine

Abstract

The intestinal fatty acid-binding protein (I-FABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in the uptake of dietary fatty acids and their intracellular transport. In this study, the full-length cDNA of I-FABP was cloned from pig intestine by homology cloning approach combined with 3′ and 5′ RACE. Sequence analysis and bioinformatics study showed that this cDNA contained 614 nucleotides, with a 399 bp open reading frame (ORF) flanked by a 43 bp 5′ UTR and a 172 bp 3′ UTR. The encoded 132 amino acids of pig I-FABP with a molecular weight of approximately 15 kDa shared a high sequence identity of 68%–85% with those of other species. In addition, the phylogenetical analysis also indicated that the pig I-FABP was in the same branch with those of other species. The tissue-specific expression of pig I-FABP was measured by Northern hybridization and semi-quantitative RT-PCR. The results demonstrated that pig I-FABP mRNA was extensively present in various tissues, but I-FABP transcript of approximately 620 bp was more abundant in intestine than in other tissues.