Abstract
The aryl hydrocarbon receptor (AhR) is a member of the basic helix–loop–helix/Per-Arnt-Sim (bHLH/PAS) family of transcription factors. In the present study, a cDNA encoding an AhR homologue was initially cloned and sequenced from the clam, Ruditapes philippinarum. The predicted amino acid sequences contain regions characteristic of AhRs from other species including basic helix–loop–helix (bHLH) and Per-ARNT-Sim (PAS) domains, but it does not contain distinct Q-rich domain as found in the mammalian AhRs. The clam AhR homologue cDNA coded for a 765 amino acids protein and phylogenetic analysis demonstrated that it was clustered within the invertebrate AhRs branch. The clam AhR homologue mRNA expression was detected in all the adult tissues tested (gill, digestive gland, adductor muscle and mantle) and highest transcription level were observed in gill compared to other tissues. Quantitative real-time RT-PCR analysis revealed that the clam AhR homologue mRNA expression levels in gill and digestive gland of R. philippinarum were induced by benzo( a)pyrene (BaP) and the absolute expression levels of the gene showed temporal and dose-dependent response.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
