Abstract
Objective To study the sequence of HFRS vaccine strain that is widely used in China. Methods The G1 genome segment of Z10 virus was amplified with RT PCR, and after being identified and purified the products were cloned into pGEM T vector. The clone was sequenced by Sanger's dideoxy chain termination method. Results 1?449 bases of Z10 G1 genome segment, coding for 483 amino acids were determined. The base compositions for the G1 segment RNA determined from cDNA sequence information were 30.8% A, 21.9% G, 19.1% C and 28.2% U. These values were similar to those of the 76/118, Lee and SR virus. As compared to Z10 G1 segment, the sequence homology at the nucleotide level was 87%(76/118, type Ⅰ), 86% (Lee, type Ⅰ),86% (Hojo, type Ⅰ), 67%(R22,type Ⅱ), and 59%(K22, type Ⅲ).The Z10 virus G1 protein had higher amino acid sequence identity with other Hantaan viruses (94% 95% homology) than Seoul type viruses (77% 80%). More amino acid sequence heterogeneity between the Z10 and 76/118 was observed in N terminal, especially cluster of divergence of ten amino acids at position 84 to 93 of the Z10 virus G1 protein. Conclusions (1) Z10 strain is one of the Hantaan viruses. (2) The important region of G1 segment of Z10 was conservative. (3) Although substantial divergence of nucleotide sequences of the G1 genome segment was found between the Z10 strain and other type I Hantaan viruses, relatively high amino acid sequence homology was shown among them. Thus, good immune protection could be obtained with the inactivated vaccine grown on Meriones unguiculatus kidney cells against other strains of Hantaan viruses.
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