Abstract
Momordica charantia (bitter melon) is widely consumed as a vegetable and as a folk medicine. Entkaurene oxidase is a key enzyme of gibberellin (GA) synthesis by controlling the early GA biosynthesis. In this study, ent-kaurene oxidase cDNA sequence was successfully amplified from the total cDNA of pistillate flower buds, and a complete open reading frame (ORF) with 1563 bp, encoding 520 amino acids, containing 151 bp of 5’-untranslated region sequences and 217 bp of the 3’-untranslated region sequences was found. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that the expression level of M. charantia ent-kaurene oxidase (McEKO) gene in pistillate flowers was significantly higher than in staminate flowers and neutral flowers, and the expression level was especially abundant in root and stem apexes. The gene was express in Escherichia coli cells with a molecular weight of 58.8 kD observed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
