Molecular cloning and functional characterization of the OCTN2 transporter at the RBE4 cells, an in vitro model of the blood–brain barrier

Abstract

The transport of l-carnitine (4- N-trimethylamino-3-hydroxybutyric acid), a compound known to be transported by the organic cation transporter/carnitine transporter OCTN2, was studied in immortalized rat brain endothelial cells (RBE4). The cells were found to take up l-carnitine by a sodium-dependent process. This uptake process was saturable with an apparent Michaelis–Menten constant for l-carnitine of 54±10 μM and a maximal velocity of 215±35 pmol/mg protein/h. Besides l-carnitine, the cells also took up acetyl- l-carnitine and propionyl- l-carnitine in a sodium-dependent manner and TEA in a sodium-independent manner. RT-PCR with primers specific for the rat OCTN2 transporter revealed the existence of OCTN2 mRNA in RBE4 cells. Screening of a cDNA library from RBE4 cells with rat OCTN2 cDNA as a probe identified a positive clone which showed, when expressed in HeLa cells, the functional characteristics of OCTN2. The HeLa cells expressing the RBE4 OCTN2 cDNA showed a sixfold increase in l-carnitine uptake and a fourfold increase in TEA uptake in a sodium-containing buffer. Typical inhibitors for organic cation transporters (e.g. MPP + or TEA) showed an inhibitory effect on the transport of l-carnitine and TEA into the transfected cells. Similarly, unlabeled l-carnitine inhibited the transport of [ 3H]- l-carnitine and [ 14C]TEA in transfected HeLa cells. It is concluded that RBE4 cells, a widely used in vitro model of the blood–brain barrier (BBB), express the organic cation/carnitine transporter OCTN2.