Abstract
SecE is an essential membrane component of theEscherichia coliprotein translocation machinery, which utilizes ATP and the proton motive force as energy sources. ThesecEhomologue of marineVibrio alginolyticus,in which protein translocation requires ATP and the sodium motive force, was cloned, sequenced, and characterized. Unlike most SecE homologues found in various organisms, SecE ofV. alginolyticus(Va-SecE) possesses three transmembrane sequences likeE. coliSecE (Ec-SecE) and complements the ΔsecEmutation ofE. coli.Alignment of the Ec-SecE and Va-SecE sequences revealed that the non-essential N-terminal half was less homologous than the essential C-terminal half between the two SecEs. TheE. colistrain was able to grow and translocate the secretory protein in the complete absence of Ec-SecE when Va-SecE was expressed. The stabilization of SecY overproduction by Va-SecE was as effective as that by Ec-SecE, indicating that Va-SecE interacts withE. coliSecY despite the difference in energy requirement.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
