Molecular cloning and expression of a new gene, GON-SJTU1 in the rat testis

Zhao-Juan Yang,Ji Wu,Shu-Qin Wang,Geng G Tian,Ning Sun

doi:10.1186/1477-7827-8-43

Zhao-Juan Yang, Ji Wu + Show 3 more

Open Access

https://doi.org/10.1186/1477-7827-8-43

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Abstract

BackgroundSpermatogenesis is a complex process involving cell development, differentiation and apoptosis. This process is governed by a series of genes whose expressions are highly regulated. Male infertility can be attributed to multiple genetic defects or alterations that are related to spermatogenesis. The discovery, cloning and further functional study of genes related to spermatogenesis is of great importance to the elucidation of the molecular mechanism of spermatogenesis. It is also physiologically and pathologically significant to the therapy of male infertility.MethodsGON-SJTU1 was identified and cloned from rat testis by cDNA library screening and 3'-and 5'-RACE. The products of GON-SJTU1 were assessed by Northern and Western blotting. The expression of GON-SJTU1 was also examined by In situ hybridization and immunohistochemistry.ResultsHere we identified and cloned a new gene, GON-SJTU1, with the biological process of spermatogenesis. GON-SJTU1 is highly expressed in the testis from day 1 to 15 and then decreased, suggesting that GON-SJTU1 might be a time-related gene and involved in the early stage of spermatogenesis. And the expression of GON-SJTU1 in the testis occurred in some male germ cells, particularly in gonocytes and spermatogonial stem cells.ConclusionGON-SJTU1 may play a role in the biological process of spermatogenesis.

Highlights

Spermatogenesis is a complex process involving cell development, differentiation and apoptosis
We recently demonstrated the importance of short-type PB-cadherin (STPB-C) in gonocyte survival, proliferation and selfrenewal of spermatogonial stem cells (SSCs) [6,7,8]
* Correspondence: jiwu@sjtu.edu.cn 1 School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China † Contributed Full list of author information is available at the end of the article over, we found that STPB-C promoted self-renewal of SSCs via activating Janus kinase/signal transducer and activator of transcription (JAK-STAT) and phosphoinositide-3 kinase (PI3-K)/Akt, and blocking transforming growth factor (TGF)-β1 signaling [8]

Summary

Introduction

Spermatogenesis is a complex process involving cell development, differentiation and apoptosis. The discovery, cloning and further functional study of genes related to spermatogenesis is of great importance to the elucidation of the molecular mechanism of spermatogenesis. It is physiologically and pathologically significant to the therapy of male infertility. With the increase of infertility rates in couples, the cellular and molecular mechanisms involved in spermatogenesis have held the interest of many biologists and researchers This complex process involves cell development, differentiation and apoptosis. The spermatogonia form the resident stem cell It constantly divides by mitosis and differentiates through meiosis and spermiogenesis to form spermatocytes, spermatids and spermatozoa [5].

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