Molecular cloning and expression characterization of flavonol synthase genes in peanut (Arachis hypogaea)

Shunli Cui,Mingyu Hou,Guojun Mu,Xinlei Yang,Yongjiang Zhang,Lifeng Liu

doi:10.1038/s41598-020-74763-w

Shunli Cui, Mingyu Hou + Show 4 more

Open Access

https://doi.org/10.1038/s41598-020-74763-w

Copy DOI

Journal: Scientific Reports	Publication Date: Oct 19, 2020
Citations: 10	License type: open-access

Affiliation: Hebei Agricultural University

Abstract

Flavonol is an important functional bioactive substance in peanut seeds, and plays important roles responding to abiotic stress. The flavonol content is closely related to the activity and regulation of gene expression patterns of flavonol synthase (FLS). In this study, eight FLS genes, AhFLSs were cloned and their expression characterization in different peanut organ and seedling under different abiotic stress were conducted. The results showed that the expressions levels of AhFLSs were differed in all assayed peanut organs and seedlings under abiotic stress treatments. Expression levels of AhFLS2, AhFLS3, AhFLS4, and AhFLS6 were higher than those of other AhFLSs. The flavonol contents of peanut organs and seedlings under different abiotic stress were also determined using high performance liquid chromatography (HPLC). Dried mature peanut seeds were the organ tissue with the highest flavonol content, and flavonol content increased with seed development. Under abiotic stress treatments, the types of flavonols induced differed among stress treatments. Correlation analysis results suggested that eight AhFLS genes may have different functions in peanut. Moreover, changes in the expression of the eight genes appear to has substrate preference. These results can lay the foundation for the study of improving nutritional value of peanut seed and resistance of peanut plant.

Highlights

Flavonol is an important functional bioactive substance in peanut seeds, and plays important roles responding to abiotic stress
It was found that the leaves and flowers of S. baicalensis contained more myricetin, while kaempferol content was highest in roots, but with quercetin as the main flavonol in s tems[17]
Wang et al.[3] showed that quercetin is the main component among five flavonoids in peanut seeds, namely daidzein, genistein, myricetin, quercetin, and kaempferol

Summary

Introduction

Flavonol is an important functional bioactive substance in peanut seeds, and plays important roles responding to abiotic stress. The flavonol content is closely related to the activity and regulation of gene expression patterns of flavonol synthase (FLS). Eight FLS genes, AhFLSs were cloned and their expression characterization in different peanut organ and seedling under different abiotic stress were conducted. The results showed that the expressions levels of AhFLSs were differed in all assayed peanut organs and seedlings under abiotic stress treatments. Expression levels of AhFLS2, AhFLS3, AhFLS4, and AhFLS6 were higher than those of other AhFLSs. The flavonol contents of peanut organs and seedlings under different abiotic stress were determined using high performance liquid chromatography (HPLC). Changes in the expression of the eight genes appear to has substrate preference These results can lay the foundation for the study of improving nutritional value of peanut seed and resistance of peanut plant. Chromosome A02 B02 A10 A05 A05 A05 A05 B05 A10 B10 A10 B10 A08 B07 A09 B09 A07 B07

Objectives

Methods

Results

Conclusion