Abstract
We report the cloning of two novel rat regulators of G-protein signaling (RGS) cDNAs using a degenerate PCR strategy. TherRgs12andrRgs14cDNAs encode predicted polypeptides of 1387 and 544 amino acids, respectively. We have also identified the human orthologue ofrRgs12by alignment of cosmid sequences in the database which map the humanRGS12gene to chromosome 4p16.3. Furthermore, we identified human ESTs with high homology torRgs14which map to human chromosome 5qter. Northern blot analysis indicates thatrRgs14is expressed at high levels in brain, lung, and spleen, whereasrRgs12is expressed at high levels in brain and lung and lower levels in testis, heart, and spleen. Analysis of the predicted rRGS12 and rRGS14 polypeptides indicates that they are closely related and possess regions of homology outside of the conserved RGS domain. We have also identified conserved regions in RGS12 which are similar to protein domains found in mouse rhophilin and coiled-coil proteins suggesting possible interactions with ras-like G-proteins.
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