Abstract
Banana cultivars may experience chilling or freezing injury in some of their cultivated regions, where wild banana can still grow very well. The clarification of the cold-resistant mechanism of wild banana is vital for cold-resistant banana breeding. In this study, the central stress integrator gene KIN10 and some cold-acclimation related genes (HOS1 and ICE1s) from the cold-resistant wild banana ‘Huanxi’ (Musa itinerans) were cloned and their expression patterns under different temperature treatments were analyzed. Thirteen full-length cDNA transcripts including 6 KIN10s, 1 HOS1 and 6 ICE1s were successfully cloned. Quantitative real-time PCR (qRT-PCR) results showed that all these genes had the highest expression levels at the critical temperature of banana (13 °C). Under chilling temperature (4 °C), the expression level of KIN10 reduced significantly but the expression of HOS1 was still higher than that at the optimal temperature (28 °C, control). Both KIN10 and HOS1 showed the lowest expression levels at 0 °C, the expression level of ICE1, however, was higher than control. As sucrose plays role in plant cold-acclimation and in regulation of KIN10 and HOS1 bioactivities, the sucrose contents of wild banana under different temperatures were detected. Results showed that the sucrose content increased as temperature lowered. Our result suggested that KIN10 may participate in cold stress response via regulating sucrose biosynthesis, which is helpful in regulating cold acclimation pathway in wild banana.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-015-1617-z) contains supplementary material, which is available to authorized users.
Highlights
Banana (Musa spp.) is one of the most important nutrient-rich crops, staple foods and ornamental plants cultivated in tropical and subtropical regions where temperature is relatively high
In total, 6 SNF1-related protein kinase catalytic subunit alpha 10 (KIN10s) (KIN10-1–KIN10-6), 1 HOS1 and 6 Inducer of CBF expression 1 (ICE1) (ICE1-1–ICE1-6) genes were successfully cloned from cold-resistant wild banana ‘Huanxi’
Identification, characterization and bioinformatic analysis of KIN10 genes from cold‐resistant wild banana ‘Huanxi’ Multiple-sequence BLAST search revealed that KIN101, KIN10-2 and KIN10-3 had similar ORF sequences that were 95.51 % identical to the KIN10 of Malaysian wild banana (Musa acuminata) (GSMUA_Achr10 G09220_001)
Summary
Banana (Musa spp.) is one of the most important nutrient-rich crops, staple foods and ornamental plants cultivated in tropical and subtropical regions where temperature is relatively high. Considerable interests still exit in exploring banana cold-resistant genes and developing cold tolerant banana cultivars due to the chilling or freezing injuries they might experience at some of their cultivated regions (Yang et al 2012). It was reported that sucrose can enhance cold hardening of plants by regulating expression of cold-acclimation-associated genes such as CBF (C-repeat/DRE-binding factor), ICE1 (inducer of CBF expression 1), HOS1 (high expression of osmotically responsive gene 1) and so on (Palonen and Junttila 2002; Rekarte-cowie et al 2008). Its CBF AP2 motif combines with the CRT/ DRE element in the COR promoter to enhance COR expression and plant cold tolerance (Chen et al 2014). In Arabidopsis, three CBF genes, CBF1, CBF2, and CBF3, were found (Stockinger et al 1997; Liu et al SpringerPlus (2015) 4:829
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