Abstract
A ColE1 hybrid, pB2, carrying the structural gene for the valyl-tRNA synthetase, valS, and the pyrBI operon was isolated from the Clarke-Carbon collection. Strain GRB238 with a temperature-sensitive mutation in the valS gene showed a three-fold increase in valyl-tRNA synthetase activity after transformation with pB2. The valS gene was subcloned into pBR322 after partial Sau3A digestion of pB2. The new plasmid, pHOV1 had a 9.4-kb insert in pBR322 and cells carrying pHOV1 showed an eight-fold increase in specific valyl-tRNA synthetase activity. The localization and direction of transcription of valS in pHOV1 was determined by transposon-directed mutagenesis and analysis of protein synthesis in minicells. The valS gene was shown to be transcribed opposite to the direction of DNA replication i.e., counterclockwise.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
