Molecular cloning and characterization of retinoid X receptor and ecdysone receptor from the lobster, Homarus americanus

Abstract

Event Abstract Back to Event Molecular cloning and characterization of retinoid X receptor and ecdysone receptor from the lobster, Homarus americanus Shirley Tiu1* and Stephen S. Tobe1 1 University of Toronto, Department of Cell and Systems Biology, Canada Two cDNAs encoding retinoid X receptor (HaRXR) and ecdysone receptor (HaEcR) were cloned and sequenced from the lobster Homarus americanus by PCR cloning techniques. The amino acid sequences of both HaRXR and HaEcR are highly homologous with those of other crustacean and insect, and also showed moderate homology to those of vertebrates. Both HaRXR and HaEcR show highest gene expression in mandibular organ (MO) in both adult male and female lobster, and showed a different expression pattern in different regions of MO. Farnesoic acid (FA) and methyl farnesoate (MF) biosynthesis rate are different in different regions of MO, which is coincided to the gene expression profile of HaRXR in MO. Different regions of MO were treated by 20-hydroxyecdysone (20-HE) and semi-qualitative PCR was performed to study the effect of 20-HE on HaRXR and HaEcR gene expression. Opposite to the stimulatory effect of 20-HE on HaRXR and HaEcR in hepatopancreas and ovary, 20-HE has an inhibitory effect on HaRXR and HaEcR in MO. Furthermore, 20-HE also inhibits FA and MF biosynthesis in MO. Hence, 20-HE may play an important role in MF metabolism, and regulating expression of different genes by binding of RXR/EcR in crustacean. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Tiu S and Tobe SS (2010). Molecular cloning and characterization of retinoid X receptor and ecdysone receptor from the lobster, Homarus americanus. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00035 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Shirley Tiu, University of Toronto, Department of Cell and Systems Biology, Toronto, Canada, shirley.tiu@utoronto.ca Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Shirley Tiu Stephen S Tobe Google Shirley Tiu Stephen S Tobe Google Scholar Shirley Tiu Stephen S Tobe PubMed Shirley Tiu Stephen S Tobe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.