Molecular cloning and characterization of low-molecular-weight glutenin sequences from an old Hungarian wheat variety, Bánkúti 1201

Abstract

The old Hungarian wheat variety Bank´uti 1201 was widely grown in Hungary mainly because of its outstanding breadmaking quality. In order to isolate and preserve the genes of this remarkable variety a cDNA library wascreated from the immature kernels of a selected line of Bank´uti 1201.When the library was screened a large number of LMW glutenin-specific clones were isolated and of these eight genes were finally sequenced. The sequence alignments revealed several new LMW glutenin genes. The results obtained so far suggest that this old variety may carry many other unknown storage protein genes of either theoretical or commercial importance.