Abstract
Background Clonorchis sinensis causes clonorchiasis, a potentially serious disease. Growth factor receptor-bound protein 2 (Grb2) is a cytosolic protein conserved among animals and plays roles in cellular functions such as meiosis, organogenesis and energy metabolism. In the present study, we report first molecular characters of growth factor receptor bound-protein (CsGrb2) from C. sinensis as counter part of Grb2 from animals and its possible functions in development and organogenesis of C. sinensis.Methodology/Principal FindingsA CsGrb2 cDNA clone retrieved from the C. sinensis transcriptome encoded a polypeptide with a SH3-SH2-SH3 structure. Recombinant CsGrb2 was bacterially produced and purified to homogeneity. Native CsGrb2 with estimated molecular weight was identified from C. sinensis adult extract by western blotting using a mouse immune serum to recombinant CsGrb2. CsGrb2 transcripts was more abundant in the metacercariae than in the adults. Immunohistochemical staining showed that CsGrb2 was localized to the suckers, mesenchymal tissues, sperms in seminal receptacle and ovary in the adults, and abundantly expressed in most organs of the metacercariae. Recombinant CsGrb2 was evaluated to be little useful as a serodiagnostic reagent for C. sinesis human infections.ConclusionGrb2 protein found in C. sinensis was conserved among animals and suggested to play a role in the organogenesis, energy metabolism and mitotic spermatogenesis of C. sinensis. These findings from C. sinensis provide wider understanding on diverse function of Grb2 in lower animals such as platyhelminths.
Highlights
Clonorchiasis sinensis (C. sinensis) presents a serious health problem that is epidemic in East Asian countries, such as, China, Korea, Vietnam and Thailand
Growth factor receptor-bound protein 2 (Grb2) protein found in C. sinensis was conserved among animals and suggested to play a role in the organogenesis, energy metabolism and mitotic spermatogenesis of C. sinensis
We describe the molecular character of CsGrb2 and its possible function in C. sinensis organogenesis and development
Summary
Clonorchiasis sinensis (C. sinensis) presents a serious health problem that is epidemic in East Asian countries, such as, China, Korea, Vietnam and Thailand. It has been reported that about 35 million people were infected worldwide [1]. Mammals are the final hosts of C. sinensis and are infected by consuming raw or uncooked freshwater fish containing metacercariae. Clonorchis sinensis causes clonorchiasis, a potentially serious disease. Growth factor receptor-bound protein 2 (Grb2) is a cytosolic protein conserved among animals and plays roles in cellular functions such as meiosis, organogenesis and energy metabolism. We report first molecular characters of growth factor receptor bound-protein (CsGrb2) from C. sinensis as counter part of Grb from animals and its possible functions in development and organogenesis of C. sinensis
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