Abstract
Complementary DNA (cDNA) clones encoding a transcription factor S-II were isolated and characterized. The primary structure of S-II was determined by nucleotide sequence analysis of these clones. The predicted primary structure was consistent with the model that we proposed previously from the results of biochemical analyses of S-II. Using these clones as probes, we analyzed the mRNA for S-II. RNA blot analysis demonstrated the presence of four species of mRNA that hybridized with S-II cDNA in Ehrlich ascites tumor cells. This is the first evidence of polymorphism of mRNA encoding a transcription factor of RNA polymerase II. The results of analysis of the genomic structure suggested that the polymorphism of mRNA may be due to alternative splicing, or differences in initiation or termination of transcription.
Highlights
ComplementaryDNA clones encoding a transcription factor S-I1 were isolated and characterized
Most structure suggested that the polymorphism of mRNA of these factors appear to activate transcripbtyiosntimulating may be due to alternative splicing, or differences in the rateof transcription initiation
T o determine which met codons are used as initiationcodons for S-11, wecompared the proteins synthesizferdom cDNA in oitro with authentic S-I1 purified from Ehrlich ascites tumor cells
Summary
ComplementaryDNA (cDNA) clones encoding a transcription factor S-I1 were isolated and characterized. We screened a cDNA library of Ehrlich ascites tumorcells using anoligonucleotide probecorresponding to the partial amino acid sequence of s-11, and isolated We analyzed the mRNA forS-I1 in Ehrlich ascites tumor cells and the genomic sequence of the S-I1gene using these clones as probes, anddemonstratedthe presence of
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