Abstract
The Beta-actin gene promoter amplified by Polymerase chain reaction (PCR) technique taking genomic DNA of Seabass (Lates calcarifer) as template and the size of Seabass Beta-actin gene promoter was confirmed around 2.2 kb on Agarose gel. The amplified PCR product was ligated to linear vector pTZ57R/T (2.886 kb) and transformed into Escherichia coli DH5α cells with an efficiency of 1 × 105 transformants/μg plasmid. On cleavage with BamHI and XbaI, the desired size of insert (2.2 kb) excise out, which was further confirmed by PCR taking recombinant plasmid DNA as template. Thus, this information on Beta-actin gene promoter of Lates calcarifer is further useful to study gene expression and construction of all fish gene transfer vector to produce auto-transgenic fish for sustainable and profitable aquaculture production.
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