Abstract
To elucidate the effect of anthocyanidin synthase (ANS) gene on anthocyanin accumulation in fruit skin of Prunus persica (L.) Batsch cv. 'Chunmei', this study cloned and characterized an ANS gene (PpANS) from peach. PpANS (GenBank accession No. KX760117) was encoded by a 1074 bp-long open reading frame (ORF) corresponding to a polypeptide consisting of 358 amino acids with a molecular mass of 40.45 kD and an isoelectric point of 5.46. PpANS contains a conserved 2-oxoglutarate- and iron-dependent dioxygenases and non-haem dioxygenase binding regions. PpANS shared high similarities to angiosperm ANS and displayed the closest genetic relationship to Prunus domestica. Real-time PCR analysis indicated that PpANS was highly expressed in fruit skin, flesh and flowers, and peach fruit skin showed the highest transcript level of PpANS. Anthocyanin accumulation analysis indicated that it was highly accumulated in fruit skin and flesh of peach. Changes in the transcript level were highly correlated with anthocyanin content in the different tissues of peach. Prokaryotic expression analysis showed PpANS that protein can be expressed correctly in E. coli, and the size of PpANS recombinant protein was consistent with its predicted size. In vitro enzyme activity assay revealed that recombinant PpANS protein could catalyze the formation the cyanidin from leucocyanidin. These results indicated that PpANS was responsible for anthocyanin accumulation in P. persica.
Highlights
IntroductionIn terms of land area for production, peach is the third most cultivated fruit crop in temperate regions (Dirlewanger et al, 2002)
Peach (Prunus persica (L.) Batsch) is a member of family Rosaceae
We identified the products of the incubation of recombinant present study reports on one ANS gene (PpANS) protein with 3,4-cisleucocyanidin as substrate in vitro through high-performance liquid chromatography (HPLC)
Summary
In terms of land area for production, peach is the third most cultivated fruit crop in temperate regions (Dirlewanger et al, 2002). External quality is mainly determined by fruit size and skin color. The most important quality is skin color, which is perceived by consumers. Skin coloration is possibly the index that determines whether consumers will purchase a given batch of peaches. For this reason, skin color has been significantly improved in the past decades, and peach breeders have selected primarily for intense red color at early stage of maturity (Iglesias and Echeverría, 2009)
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