Molecular cloning and characterization of active truncated dextransucrase from Leuconostoc mesenteroides B-1299CB4

Abstract

The open reading frame of dsrE563, a dextransucrase gene obtained from a constitutive mutant (CB4-BF563) of Leuconostoc mesenteroides B-1299, consists of 8,511 bp encoding 2,836 amino acid residues. DsrE563 contains two catalytic domains (CD1 and CD2). Two truncated derivative mutants DsrE563ΔCD2ΔGBD (DsrE563-1) and DsrE563ΔCD2ΔVR (DsrE563-2) of DsrE563 were constructed and expressed using the pRSETC vector in Escherichia coli. The derivatives DsrE563-1 (deletion of 1,620 amino acids from the C-terminus) and DsrE563-2 (deletion of 1,258 amino acids from the C-terminus and 349 amino acids from the N-terminus) were expressed as active enzymes. Both enzymes synthesized less-soluble dextran, mainly containing α-1,6 glucosidic linkage. The synthesized less-soluble dextran also had a branched α-1,3 linkage. DsrE563-2 showed 4.5-fold higher dextransucrase activity than that of DsrE563-1 and showed higher acceptor reaction efficiency than that of dextransucrase from L. mesenteroides 512 FMCM when various mono or disaccharides were used as acceptors. Thus, the glucan-binding domain was important for both enzyme expression and dextransucrase activity.