Abstract
More than 150 types of Human papillomaviruses (HPVs) have been isolated from numerous cutaneous and/or mucosal lesions. Flat wart samples on the face from 36 immunocompetent patients were collected and screened for HPV. From one sample, we cloned a putative novel genotype. The novel type consisted of 7779 bp in length with a GC content of 47.1%, containing open reading frames for putative early proteins (E1, E2, E4, E6, and E7) and two late proteins (L1 and L2). Homology searches and phylogenetic analyses indicated that it belonged to Alphapapillomavirus (Alpha-PV) species 2 and most closely resembled HPV 3. The virus fulfilled the definition of a novel type, and was named HPV 160 by the Reference Center for Papillomaviruses. The putative E7 protein of HPV 160 as well as HPV 29, 77, and 78 contained the Leu-X-Cys-X-Glu pRB-binding motif but other Alpha-PV species 2 (HPV 3, 10, 28, 94, 117, and 125) did not have this conserved motif.
Highlights
More than 150 genotypes of Human papillomaviruses (HPVs) have been isolated from human cutaneous and mucosal lesions [1,2]
We examined 36 samples of flat wart tissues from the face, and tried to determine HPV genotypes causing the warts
HPV DNAs were detected in two samples (HPV 3) without any significant cytopathic effect (CPE), suggesting CPEs are not necessarily observed in flat wart lesions at least more than 10-years-old
Summary
More than 150 genotypes of Human papillomaviruses (HPVs) have been isolated from human cutaneous and mucosal lesions [1,2]. The introduction of rolling circle amplification (RCA) for papillomaviruses [5] resulted in a significant increase in numbers of newly isolated and characterised HPV types. Based on the large number of partial sequences reported, many additional novel HPV genotypes after full sequence analyses are expected. HPV genotypes belong to the five genera, Alpha, Beta, Gamma, Mu, and Nu according to the phylogenetic relationships of their complete L1 gene sequences [3,4]. HPV genomes with 60-70% similarities comprise different species, and less than 60% are considered different genera [4]
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