Molecular Cloning and Bioinformatics Analysis of LuxR from Vibrio harveyi HY99

Abstract

LuxR gene functions as an activator, repressor, directly and indirectly controls the 625 gene expression. The LuxR gene was encoded from Vibrio harveyi , cloned, sequenced and amplified by PCR technology. The total length of the gene is 635 bp, and encoded 211 amino acids. The physicochemical properties, protein structure and genetic evolutionary relationship LuxR of Vibrio harveyi HY99 were studied and analyzed by bioinformatics methods and tools. The phylogenetic tree was constructed using MEGA 7.0 software with Neighbour-joining method. The results showed that LuxR is a stable hydrophilic and acidic protein without a transmembrane region and a signal peptide. The evolutionary analysis showed that Vibrio campbelli and Vibrio jasicida was clustered together, which indicated that the genetic relationship between the two species was the closest LuxR contains a TetR_N superfamily conserved domain associated with cell division. The 3D structure model of LuxR subunit was simulated by SWISS-MODEL software and was found that the LuxR gene belong to LuxR family transcriptional regulator. LuxR gene functions as a repressor, activator and control gene expression directly and indirectly. This conclusion could provide the basis for further research on the function and mechanism of LuxR related protein, so as to lay a foundation for understanding the mechanism of the osmotic adjustment of Vibrio harveyi .