Abstract
Cultivar ‘Kinaya’ is a melon produced from breeding between ‘Sonya’ and ‘Kinanti’. This study on phenotypic and molecular characters is carried out to support ‘Kinaya’ as a superior cultivar. Our study aimed to analyze the genetic variation of the melon ‘Kinaya Bulat Kuning’ and ‘Kinaya Kuning Lonjong’ and its parental ‘Sonya’ and ‘Kinanti’. The research was conducted in the Greenhouse of Mutihan, D.I.Yogyakarta, and the Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Quantitative characters were determined by morphometric comparisons, fruit weights, and the number of seeds. Qualitative characters included fruit color, fruit skin color, aroma, texture, and taste. The molecular characterization method was inter simple sequence repeat (ISSR) and included DNA isolation, spectrophotometry, amplification of DNA target using PCR, and visualization of DNA target. Molecular characters were analyzed using spectrophotometry and visualization of DNA bands by electrophoresis using the MVSP 3.1 program. PCR used four random primers such as UBC 807, 809, 810, and 812, which obtained 11 polymorphic and 12 monomorphic DNA bands with a polymorphism rate of 47.8%. It is known that the cultivar ‘Kinaya’ has a similarity of 72% with its inductees ‘Sonya’ and ‘Kinanti’.
Highlights
Melon (Cucumis melo L.) is a horticulture plant that belongs to the Cucurbitaceae family that grows widely in Asia and Middle East countries and is consumed in large quantities as a traditional diet (Paris et al, 2012; Shin et al, 2019; Gómez-García et al, 2020)
‘Kinaya’ is one of the new melon cultivars developed by the Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
‘Kinaya’ is the third derivative of the ‘Kinanti’ and ‘Sonya’, which was cultivated by the Laboratory of Genetic and Breeding, Faculty of Biology, Universitas Gadjah Mada
Summary
Melon (Cucumis melo L.) is a horticulture plant that belongs to the Cucurbitaceae family that grows widely in Asia and Middle East countries and is consumed in large quantities as a traditional diet (Paris et al, 2012; Shin et al, 2019; Gómez-García et al, 2020). ‘Kinaya’ is one of the new melon cultivars developed by the Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Research on genetic traits supports ‘Kinaya’ as a superior melon cultivar. ‘Kinaya’ is the third derivative of the ‘Kinanti’ and ‘Sonya’, which was cultivated by the Laboratory of Genetic and Breeding, Faculty of Biology, Universitas Gadjah Mada. Variations in melon plants consist of phenotype and genetic variations. Phenotype variations can be observed from morphological characters that appear qualitatively and quantitatively, while genetic variations can be seen through DNA analysis (Liu et al, 2013; Granier & Vile, 2014; Nadeem et al, 2017). Using ISSR, we have successfully analyzed the genetic variation of C. melo L. The present study aimed to verify genetic variations and similarity index based on ISSR molecular markers. This study will help devise future hybrid breeding and conservation approaches
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