Abstract
Background Litopenaeus Vannamei, well known as pacific white shrimp, is the most popular shrimp in the world shrimp market. Identification and characterization of shrimp muscle regulatory genes are not only important for shrimp genetic improvement, but also facilitate comparative genomic tools for understanding of muscle development and regeneration.Methodology/Principal FindingsA novel mRNA encoding for a putative DNA-binding protein LvDBP23 was identified from Litopenaeus vannamei abdominal muscle cDNA library. The LvDBP23 cDNA contains 639 nucleotides of protein-coding sequence with deduced 212 amino acids of predicted molecular mass 23.32 kDa with glycine-rich domain at amino acid position 94–130. The mRNA sequence is successfully used for producing LvDBP23 recombinant protein in sf9 insect cell expression system. The expression of LvDBP23 mRNA is presented in abdominal muscle and swimming leg muscle, as well as other tissues including intestine, lymphoid and gill. The mRNA expression has the highest level in abdominal muscle in all tested tissues. LVDBP23 transcript during the molt cycle is highly expressed in the intermolt stage. In vitro nucleic acid-binding assays reveal that LvDBP23 protein can bind to both ssDNA and dsDNA, indicating its possible role of regulation of gene transcription.Conclusions/SignificanceWe are the first to report a DNA-binding protein identified from the abdominal muscle tissue of marine shrimp L. Vannamei. Its high-level specific expression during the intermot stage suggests its role in the regulation of muscle buildup during the growth phase of shrimp molt cycle.
Highlights
Litopenaeus Vannamei, well known as pacific white shrimp, is the most popular shrimp in the world shrimp market [1]
LvDBP23 cDNA and Amino Acid Sequences analysis By using degenerated oligonucleotide DNA probes, we have identified muscle regulatory genes from L. vannamei muscle cDNA library
The mRNA sequence of LvDBP23 consists of 838 nucleotides, including an ORF of 639 nucleotides, 39 UTR of 183 nucleotides with the stop codon (TAA) and polyadenylation signal of CATAAA sequence (Fig. 1)
Summary
Litopenaeus Vannamei, well known as pacific white shrimp, is the most popular shrimp in the world shrimp market [1]. Conclusions/Significance: We are the first to report a DNA-binding protein identified from the abdominal muscle tissue of marine shrimp L. Its high-level specific expression during the intermot stage suggests its role in the regulation of muscle buildup during the growth phase of shrimp molt cycle.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.