Abstract
Here we present a comprehensive molecular mapping of virus-induced autoimmune B cell responses obtained by serological identification of antigens by recombinant expression cloning analysis. Immunoscreening of cDNA expression libraries of various organs (lung, liver, and spleen) using sera from mice infected with cytopathic (vaccinia virus [VV]) or noncytopathic (lymphocytic choriomeningitis virus [LCMV]) viruses revealed a broad specificity of the elicited autoantibody response. Interestingly, the majority of the identified autoantigens have been previously described as autoantigens in humans. We found that induction of virus-induced autoantibodies of the immunoglobulin G class largely depends on the CD40‐CD40L-mediated interaction between T and B cells. Furthermore, antibody titers against a number of autoantigens were comparable to the concomitantly induced antiviral antibody response. Comparison of serum reactivity against a selected panel of autoantigens after infection with VV, LCMV, or vesicular stomatitis virus showed that the different virus infections triggered distinct autoantibody responses, suggesting that virus infections may leave specific “autoantibody fingerprints” in the infected host.
Highlights
Molecular characterization of virus-induced autoantibody responses P Krebs*1,2, B Ludewig1,2, Metters1, Ö Türeci3 and U Sahin3
We present here a comprehensive molecular mapping of virus-induced autoimmune B cell responses obtained by SEREX analysis
Immunoscreening of cDNA expression libraries of various organs using sera from mice infected with cytopathic or non-cytopathic viruses revealed a broad specificity of the elicited autoantibody response
Summary
Molecular characterization of virus-induced autoantibody responses P Krebs*1,2, B Ludewig1,2, Metters1, Ö Türeci3 and U Sahin3. Address: 1Institute of Experimental Immunology, Department of Pathology, University Hospital Zürich, CH-8091 Zürich, Switzerland, 2Research Department, Kantonal Hospital St. Gallen, 9007 St. Gallen, Switzerland and 3III Department of Internal Medicine, Johannes Gutenberg University Mainz, D-55131 Mainz, Germany
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
