Molecular characterization of virulence genes in poultry-originated Salmonella Enteritidis and Salmonella Typhimurium

Abstract

Salmonella Enteritidis and Salmonella Typhimurium are the most common serovars observed in human salmonellosis while contaminated poultry products are the major source of Salmonella transmission to humans. Therefore, high pathogenicity of poultry-originated S. Enteritidis and S. Typhimurium strains poses a serious risk to human health. This study analyzed the virulence genes of broiler chicken-originated S. Enteritidis and S. Typhimurium strains. SipA, sipD, sopB, sopD, sopE, sopE2, sitC, sifA, ssaR, spvC and pefA genes were investigated in a total of 137 strains consisting of 105 S. Enteritidis and 32 S. Typhimurium. Nine strains (6.6%) had all genes. No negative strain was detected for all genes. SopE was found in all strains (100%). SitC (89.1%), ssaR (83.9%), sipA (70.1%), sipD (73.0%), sopE2 (68.6%), spvC (68.6%) and pefA (73.0%) were also highly prevalent. Noticeable differences were observed between serovars in terms of sopE2, spvC and pefA prevalence: 77.1%, 80% and 82.9%, respectively, of S. Enteritidis strains were sopE2, spvC and pefA positive while 40.6%, 31.3% and 40.6% of S. Typhimurium strains were positive. This finding indicates that S. Enteritidis is more frequent than S. Typhimurium in poultry populations thanks to higher virulence. Based on virulence gene distribution, the strains were divided into 44 different virulence genotypes, with the major genotype 4 (15.3%) carrying 8 of the 11 genes. The majority of strains (75.9%) were positive for at least 6 genes. S. Enteritidis and S. Typhimurium strains were highly virulent and pose a threat as a zoonotic infection.