Molecular Characterization of Vibrio Spp Isolated from Fresh Water Fish in Kafer Elsheikh Government

Abstract

The most important threatening disease problem facing freshwater aquaculture is Vibriosis that cause water-borne diseases and foodborne gastroenteritis resulted from ingesting of uncooked or undercooked fish products. Also, it is one of the most zoonotic disease which transmitted to human from fish and another aquaculture. This study aimed to isolate different vibrio species from freshwater fish. Microbiological identification of Vibrio species were made using colonial morphology, biochemical characteristics and Identification system for Gram negative bacteria (GN 24) (Diagnostic sro kits) from total of 100 clinically diseased fish samples (Oreochromis niloticus) were collected alive from two various fish farms ( each 50 fish from one farm) from Kafrelsheik Governorate., the prevalence of Vibrio species were noticed in samples tilabia (23/100) 23% which V. parahaemolyticus was found in 6/100 (6%) and V. alginolyticus was detected in 16/100 (16%) and in V. cholera 1/100 (1%), Also we used PCR using 16S rRNA gene-specific for the genus Vibrio , detection of virulence and antibacterial resistance associated genes in v. parahaemolyticus and V. alginolyticus which the most pathogenic vibrio species .The present study also focused on the emergence of Multiple Antibiotic Resistance (MAR) index with the aim of developing strategies to mitigate the potential for infection and ensure effective treatment in the near future.