Molecular characterization of Vasa homolog in the pen shell Atrina pectinata: cDNA cloning and expression analysis during gonadal development.

Abstract

Vasa is an ATP-dependent RNA helicase of the DEAD (Asp-Glu-Ala-Asp) box family and a representative component of the germ plasm. In this study, we cloned the full-length vasa homolog in the bivalve Atrina pectinata (psvasa), and performed phylogenetic analysis, mRNA expression analysis for tissue-specific distributions, and immunostaining analysis to reveal its histological localization. The sequence of psvasa was 3587bp in length and contained a 5' untranslated region of 150bp, an open reading frame of 2214bp, and a 3' untranslated region of 1223bp. The deduced amino acid sequence of psvasa was 737 amino acids long and contained evolutionarily conserved sequences reported in other animals. The mRNA expression analysis showed the highest expression levels in the gonads. Expression was especially high in the ovaries, followed by the testes. The immunostaining analysis showed Vasa-positive cells in the developing gonads, suggesting the presence of putative germ stem cells contributing to the supply of germ cells. Furthermore, characteristic Vasa signals were observed in the basophilic nuclei of the oocytes, suggesting that psvasa plays an important role in the progression of meiosis in oocytes.