Abstract
Amylases and lipase are among the most important enzymes nowadays in biotechnology. The current study was focused on the partial characterization of extracellular amylase and lipase enzymes produced by two potent isolates which previously isolated from hot spring, Ras-Sedr, south Saini, Egypt. Morphological, biochemical and molecular identification of these potent isolates were also performed. The study revealed that these isolates are belonging to actinobacteria of genus Thermoactinomyces thalpophilus KCTC 9789 and bacilloid bacteria of genus Bacillus licheniformis WSE-KSU301. The effect of physical conditions was also investigated maximum production of the tested enzymes. Crude enzymes were extracted and subjected to different temperature and pHs to measure their effect on the activity and stability of enzymes. The data obtained proved the high stability of these enzymes to high temperature up till 85oC with optimal temperature at 65oC and 55oC, for amylase and lipase, respectively. However, for pHs slightly acidic to neutral displayed the optimal pH for maximum activity for amylase and lipase, respectively. To increase the activity and stability of these enzymes, for industrial large scale production, further studies are in need.
Highlights
Bioprospecting is defined as biodiversity discovery of unique ecosystems expressing their biodiversity, microorganisms and flora including commercially valuable biological and genetic resources to accomplish financial and environmental goals [8]
The current study was focused on the partial characterization of extracellular amylase and lipase enzymes produced by two potent isolates which previously isolated from hot spring, Ras-Sedr, south Saini, Egypt
The results showed the highest sequence similarity species is (87.68%) of Thermoactinomyces thalpophilus with actinobacterial isolate (HRS-a) and of Bacillus licheniformis WSE-KSU301 (90.04%) for bacterial isolate (HRS-b)
Summary
Bioprospecting is defined as biodiversity discovery of unique ecosystems expressing their biodiversity, microorganisms and flora including commercially valuable biological and genetic resources to accomplish financial and environmental goals [8]. We presently recognize that certain microorganisms, including extremophiles, can produce enzymes which could be stable and operate under harsh conditions [9, 29]. Due to the extreme and frequently inaccessible habitats from which they emerge, problems in the isolation of thermophiles and the development of these microorganisms under laboratory conditions presented considerable limitations for their study and thereby for relevant biotechnological an industrial applications. In the late seventies and through early nineties, many widely recognized and investigated thermophiles were isolated [28]. It is not surprising, given the usefulness of thermostable enzymes in molecular biological laboratory techniques, that they were suggested as strong resources for industrial catalysis [30]. Characterization of targeted enzymes, based on physiological characters, was performed
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