Abstract
Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.
Highlights
The use of Lucilia sericata (L. sericata) blowfly larvae in diabetic wound healing have previously been reported [1]
We identified the coding sequence (CDS) of netrin-1 UNC-5 receptor in L. sericata
We identified the CDS of netrin-1 UNC-5 receptor in L. sericata which could be involved in research activities applied in the design of a new essential component in diabetic wound healing
Summary
The use of Lucilia sericata (L. sericata) blowfly larvae in diabetic wound healing have previously been reported [1]. It was shown that the netrin-1 UNC-5 receptor is expressed in capillaries More studies on this new group of molecules will be interesting. This study was conducted to identify and characterize the netrin-1 UNC-5 receptor in larvae of L. sericata This blowfly is a species in the family Calliphoridae, Class Insecta with a critical role in maggot therapy [23]. Repair of the damaged nerve tissue, the identification of the nucleic acid sequence of the UNC-5 receptor of L.sericata and its recombinant expression in the future can be effective in producing drugs for the treatment of ulcers In this way, using the recombinant protein, it increases the netrin-1 secretion and accelerates the recovery of the damaged nerve tissue
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