Molecular characterization of the [formula omitted] J558 family of heavy chain variable region gene segments

Abstract

The nucleotide sequences of the coding as well as the flanking regions of 11 A J J558 heavy chain variable region ( V H) gene segments are presented. Among these J558 V H segments was the unrearranged germline V H gene segment recruited in the predominant A strain-specific anti-arsonate response. Three other V H gene segments that are greater than 92% related to the p-azophenylarsenate (Ars) A V H gene segment were also isolated. Detailed analysis of the nucleotide sequences of these as well as the remaining seven J558 V H gene segments reveal that the J558 V H gene family is composed of distinct, but related, J558 V H subfamilies. Deletion mapping analyses were used to position the Ars A V H gene segment proximally with respect to the D H− J H clusters within the J558 V H gene family and distally with respect to its own J558 subfamily. The documentation of J558 V H subfamilies is discussed in the context of J558 V H family evolution and diversification.