Molecular Characterization of the Dwarf53 Gene Homolog in Dasypyrum villosum.

Mikhail Bazhenov,Gennady Karlov,Pavel Kroupin,Anastasiya Chernook,Mikhail Divashuk

doi:10.3390/plants9020186

Abstract

The Dwarf53 (D53) gene, first studied in rice, encodes a protein that acts as a repressor of the physiological response of plants to strigolactones—substances that regulate the activity of axillary buds, stem growth, branching of roots and other physiological processes. In this work, we isolated and sequenced the homolog of the D53 gene in several accessions of the wild grass Dasypyrum villosum of different geographical origins, resulting in the discovery of large allelic variety. A molecular marker was also created that allows us to differentiate the D. villosum D53 gene from common wheat genes. Using this marker and monosomic addition, substitution and translocation wheat lines carrying the known D. villosum chromosomes, the D53 gene was localized on the long arm of the 5V chromosome.

Highlights

The D53 gene (Dwarf53, previously referred to as Dwarf-Kyushu-3, D-K-3) was first described in rice due to the short-stemmed dominant mutation resulting from radiation mutagenesis associated with the increased tillering capacity of plants [1]
The D53 gene of D. villosum was isolated by polymerase chain reaction (PCR) using primers selected on the basis of genomic sequences of common wheat
Sequencing of the PCR products obtained from the six D. villosum accessions of diverse geographical origin showed that the D53 gene is highly polymorphic in this species

Summary

Introduction

The D53 gene (Dwarf, previously referred to as Dwarf-Kyushu-3, D-K-3) was first described in rice due to the short-stemmed dominant mutation resulting from radiation mutagenesis associated with the increased tillering capacity of plants [1]. Root branching and tilling capacity are important economically valuable properties of wheat, determining its resistance to lodging, the ability to absorb nutrients from the soil and the productivity of individual plants [6,7,8].

Objectives

Results

Conclusion