Abstract
Turnip mosaic virus (TuMV) infects crops of plant species in the family Brassicaceae worldwide. TuMV isolates were clustered to five lineages corresponding to basal-B, basal-BR, Asian-BR, world-B and OMs. Here, we determined the complete genome sequences of three TuMV basal-BR isolates infecting radish from Shandong and Jilin Provinces in China. Their genomes were all composed of 9833 nucleotides, excluding the 3′-terminal poly(A) tail. They contained two open reading frames (ORFs), with the large one encoding a polyprotein of 3164 amino acids and the small overlapping ORF encoding a PIPO protein of 61 amino acids, which contained the typically conserved motifs found in members of the genus Potyvirus. In pairwise comparison with 30 other TuMV genome sequences, these three isolates shared their highest identities with isolates from Eurasian countries (Germany, Italy, Turkey and China). Recombination analysis showed that the three isolates in this study had no “clear” recombination. The analyses of conserved amino acids changed between groups showed that the codons in the TuMV out group (OGp) and OMs group were the same at three codon sites (852, 1006, 1548), and the other TuMV groups (basal-B, basal-BR, Asian-BR, world-B) were different. This pattern suggests that the codon in the OMs progenitor did not change but that in the other TuMV groups the progenitor sequence did change at divergence. Genetic diversity analyses indicate that the PIPO gene was under the highest selection pressure and the selection pressure on P3N-PIPO and P3 was almost the same. It suggests that most of the selection pressure on P3 was probably imposed through P3N-PIPO.
Highlights
Turnip mosaic virus (TuMV) is a member of the genus Potyvirus and possesses an exceptionally broad host range in terms of plant genera and families compared to any other potyvirus; it is known to infect at least 318 species of 156 genera belonging to 43 families of plants representing many arable, vegetable and ornamental crops, especially in the family Brassicaceae [1,2]
Like the CPs of most potyviruses, these three TuMV isolates carried the 2882DAG2884 motif interacting with the PTK of HC-Pro as an important factor related to aphid transmission, and the 3054R-(X)43-D3098 motif associated with virus movement [36]
We reported the complete genome sequences of three Chinese TuMV isolates infecting Raphanus sativus that were grouped to basal-BR lineage according to their molecular characteristics
Summary
Turnip mosaic virus (TuMV) is a member of the genus Potyvirus and possesses an exceptionally broad host range in terms of plant genera and families compared to any other potyvirus; it is known to infect at least 318 species of 156 genera belonging to 43 families of plants representing many arable, vegetable and ornamental crops, especially in the family Brassicaceae [1,2]. In the study reported here, the complete genome sequences of three TuMV isolates collected from radish from Shandong and Jilin Provinces in China were determined We analyzed their phylogenetic relationships, recombination pattern and genetic diversity, together with 30 other previously reported representative TuMV isolates collected from different hosts around the world. Like the CPs of most potyviruses, these three TuMV isolates carried the 2882DAG2884 motif interacting with the PTK of HC-Pro as an important factor related to aphid transmission, and the 3054R-(X)43-D3098 motif associated with virus movement [36]. The recently described ORF coding the putative protein PIPO [6] was identified within the P3 ORF expressed by a +2 ribosomal frameshift, starting from a G1A4 motif at position 3076 (Figure 1) This motif was distinct from the highly conserved motif G1–2A6–7 that is known for other potyviruses [6] and ended with a UAA termination codon at position 3258–3261
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
