Abstract
The study was carried out to detect the identity of Proteus spp. isolated from various clinical specimens in Erbil City by polymerase chain reaction (PCR) technique. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. Fifty-one Proteus isolates, (47) Proteus mirabilis, and (4) Proteus vulgaris, were undergone PCR assay using specific primers, targeting the genes ureR and Urease C (ureC), that encode for urease enzyme as a virulence factor of Proteus species. It was found that all isolates of Proteus mirabilis yielded positive result for ureR gene with an amplicon length of 225 bp, as well as, all isolates of Proteus vulgaris exhibited positive PCR products on gel for ureC gene with an amplicon length of 263 bp. Our results indicate that ureR and ureC based PCR method seems to be an appropriate method for characterization of Proteus mirabilis and Proteus vulgaris respectively.
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