Molecular characterization of peroxisome proliferator activated receptor gamma (PPARγ) in loach Misgurnus anguillicaudatus and its potential roles in fatty acid metabolism in vitro

Abstract

Abstract Peroxisome proliferator activated receptor gamma (PPARγ) was firstly investigated in loach Misgurnus anguillicaudatus by RACE (rapid amplification of cDNA ends) and qPCR (real-time quantitative PCR). An in vitro cell culture experiment was then performed and showed that loach PPARγ positively affected expressions of four fatty acid metabolism related genes, namely fatty acid desaturase 2, lipoprotein lipase, elongase of very long chain fatty acids 5 and sterol regulatory element-binding protein-1c. At last, effects of C18:2n-6 (linoleic acid, LA) and C18:3n-3 (linolenic acid, LNA) on loach PPARγ expression were investigated. Loach fin cells cultured with 50 μM and 100 μM of LA or 100 μM LNA exhibited significantly lower PPARγ transcriptions than those cultured with 0 μM LA and LNA, showing that high-dose LA/LNA suppressed PPARγ expression in loach fin cells. Results of the two in vitro experiments suggested that PPARγ played an important role in fatty acid metabolism of the loach.