Abstract
Camellia chekiangoleosa is an economically important woody plant from the Genus Camellia in Theaceae, and its seed kernels are rich in edible oils of high health value. Yet, little is known about the molecular regulation of growth and development in C. chekiangoleosa. In this study, we characterized the MYB (Myeloblastosis) gene family that was widely involved in plant development and stress responses, and identified 235 members from the C. chekiangoleosa genome. Based on transcriptomic analysis of multiple tissues, we obtained tissue-specific expression profiles of the MYB genes. We found that 37 MYB genes were highly expressed during seed development, and among them, CcMYB33 (GAMYB) was specifically expressed in the seed coat, suggesting that it may be an important regulator. We cloned full-length sequences of the CcMYB33 gene and further analyzed its sequence characteristics and expression pattern. Our results indicated that CcMYB33 is an R2R3-type MYB transcription factor that is closely related to GAMYB genes of Arabidopsis thaliana. We showed that ectopic expression of CcMYB33 in Arabidopsis lines caused pleiotropical developmental defects, including abnormal leaves, fused stamen, and early flowering, among other things. This work identified important MYB regulators in the regulation of development and growth in C. chekiangoleosa, providing support for further molecular and genetic studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
