Molecular characterization of mung bean (Vigna radiataL.) genotypes through RAPD, ISSR and SSR markers

Abstract

The RAPD, ISSR and SSR marker system was applied to the 11 mung bean genotypes. The RAPD data showed that primer OPC-11 was the best primer resulting good amplification with maximum PIC value (0.893). Dendrogram constructed using the RAPD data clearly distinguished all genotypes. It revealed that genotypes MEHA and HUM-1 found in one cluster and shared maximum 75.00% similarity; however, genotype PUSHA VISHAL have out grouped from other 10 genotypes and shared minimum 45.5% similarity. The ISSR data showed that primer 810 was the best primer exhibit good amplification with maximum PIC value (0.907). Jaccard’s similarity coefficient ranged from 0.229 to 0.886. The ISSR results indicated that maximum similarity of 88.6% was found between IPM-2-3 and GM-4, while minimum similarity of 22.9% was obtained between PUSHA VISHAL and K-851. From the SSR data it was found that the primer VR-304 was the best primer showing good amplification with maximum PIC value (0.746). Jaccard’s similarity coefficient ranged from 0.607 to 0.952. So the SSR data revealed that MEHA showed maximum variability compared to other ten genotypes. The combined RAPD, ISSR and SSR analysis revealed that out of eleven genotypes IPM-2-3 and HUM-1 showed maximum 80.9% similarity. The lowest similarity of 40.2% was found between PUSHA VISHAL and K-851.