Abstract
BackgroundThe prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum β-lactamases have been compared. Rep-PCR was used to determine the clonal relationship of the organisms. Plasmids were classified according to their incompatibility. Class 1 and Class 2 integrons and antibiotic resistance genes were analysed by PCR and sequencing.ResultsBoth collections of organisms contained a large diversity of unrelated strains with some clones distributed in both groups of isolates. Large plasmids were identified in the two groups of organisms. Plasmids with replicons repK and repColE were more frequent among ESBL-producing isolates, while repFIA, repFII and repA/C replicons were more frequent in isolates lacking ESBL. Conjugative plasmids with repK and repA/C replicons coded for CTX-M-14 and CMY-2 β-lactamases, respectively. No significant differences were observed in the distribution of class 1 and class 2 integrons among multiresistant E. coli producing or not ESBL, and dfrA17-ant(3″)-Ie was the cassette arrangement most commonly found.ConclusionsIn the concrete temporal and geographical context of this study, multiresistant E. coli producing ESBL or other mechanisms of resistance were largely clonally diverse and present some differences in the types of harboured plasmids. Still, some clones were found in both ESBL-producing and –lacking isolates.
Highlights
The prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum β-lactamases have been compared
Extensive characterization of plasmid families has provided additional information on epidemiological aspects of transmissible antimicrobial resistance [7]. Many of these studies have focused on E. coli producing extended-spectrum beta-lactamases (ESBLs) [8,9]
The objective of this study was to determine whether the clonal variability and content of plasmids, resistance genes and integrons of clinical isolates of E. coli producing
Summary
The prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum β-lactamases have been compared. Extensive characterization of plasmid families (usually by replicon typing [4,5] or, more recently, by relaxase identification [6]) has provided additional information on epidemiological aspects of transmissible antimicrobial resistance [7]. Many of these studies have focused on E. coli producing ESBL [8,9]. Some plasmid families were demonstrated to be largely prevalent in Enterobacteriaceae, emerging in association with specific
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