Abstract
Background: Methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the leading causes of infections in animals as well as human beings and is associated with significant morbidity, mortality, length of stay and cost burden. Methods: In this study, 725 samples of nasal swabs and milk were collected randomly from cattle, buffalo, sheep and goats and these samples were inoculated on Mannitol salt agar mixed with Oxacillin Resistance Selective Supplement for the molecular characterization of MRSA isolates through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and polymerase chain reaction (PCR) test. Result: Out of 725 samples, 171 were found positive for Oxacillin resistant S. aureus. The SDS-PAGE showed different bands of molecular weight 13, 17, 20, 24, 26, 28, 33, 36, 39, 43, 47, 59, 64, 72, 86, 97 and 121 kDa. An amplified mec A DNA fragment (137) of 533 base pairs (bp) and PCR product of fem A (133 isolates) of 510 bp were detected in isolates.
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