Abstract
The study was looking to isolation and identification of various species of Malassezia isolates associated with various clinical phenomena of pityriasis versicolor by molecular Techniques the results of DNA extraction showed that most of the isolates give just single bundle of DNA on agarose gel, and according to PCR results, thirteen DNA samples (43.3%) were amplified after PCR program for amplification by two sets of primers ITS1-ITS4 3.2 and V9G-LS266, then all the thirteen isolates were subjected to DNA sequencing which showed the following results: eight isolates identified as Malassezia furfur and five as Malassezia globosa with the alignment bellow of each species. Malassezia furfur isolate POL.10.11. IIIA 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. Sequence ID: KC152898.1 Length: 848 Number of Matches: 1Range 1: 1 to 848. Phylogenetic analysis of the 13 Malassezia isolates were analyzed by MEGA 5.05 and compared with sequences of different Malassezia species available in Gen Bank database, the data showed a clear convergence between our Malassezia isolates from Basra patients and that of the Gen Bank database.
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