Abstract
A new strain of Fusarium oxysporum (Schl.) emend. Synder & Hansen, viz. Fusarium oxysporum G30, is reported from seven different decomposing vegetable wastes and a mixture of the same in equal proportions, in Imphal, Northeast India. This strain was isolated by surface sterilization technique, followed by re-culturing till pure culture was obtained. The pure culture was characterised morphologically based on the colony features: colour, texture, colony diameter; conidial features: macro and microconidial shape, size, septation, presence of chlamydospores and foot-shaped basal cells. The strain was also identified at molecular level by partial sequencing using universal primers, ITS4 and ITS5. The ITS data reveals that isolated culture ( F. oxysporum G30) match the existing isolates in Gen Bank (Gen Bank accession No. GQ497156.1) by 99% and it has got a code segment particular to this strain. This strain showed ubiquitous nature with mean percent frequency of occurrence ranging from 39.5 to 68% at various stages of decomposition of the selected vegetable wastes.
Highlights
IntroductionSynder & Hansen is an ubiquitous saprophyte found predominantly in the environment
Identifying this particular strain at molecular level will be useful for formulating effective biological treatment of vegetable wastes which have already become a problem for dumping in city areas
In both decomposition studies a particular isolate of Fusarium oxysporum G30 was obtained from all the study samples throughout the study period with mean percent frequency of occurrence (F.O. %) ranging from 39.5% (M. paradisiaca) to 68.0% (B. arundinaceae) in litter bag decomposition and 44.0% (B. arundinaceae) to 63.5% in pot decomposition (Fig. 1)
Summary
Synder & Hansen is an ubiquitous saprophyte found predominantly in the environment This species has been reported to be responsible for a number of diseases in plants. Recent studies (Sharma and Pandey, 2012) reveal that this species occurs in maximum population in decaying vegetable wastes. Identifying this particular strain at molecular level will be useful for formulating effective biological treatment of vegetable wastes which have already become a problem for dumping in city areas. Such beneficial application of this fungus has been given less attention so far. In the present study, the most common F. oxysporum strain, having the highest frequency of occurrence in decaying vegetable wastes, have been isolated and characterized at molecular level
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