MOLECULAR CHARACTERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM SOME ABATTOIRS IN DAKAHLIA, EGYPT

S Shafik ,Mahmoud A E Abdelrahman

doi:10.21608/avmj.2019.168762

Abstract

A total of 400 samples were collected from 100 slaughtered carcasses (cattle, buffalo, sheep and goat, 25 of each). Four samples from each carcass representing: muscle, liver, kidney and heart were collected for detection of L. monocytogenes. Also, 100 environmental samples representing water used in abattoir, knife swabs and swabs from abattoir walls before and after cleaning, (25 for each) and 100 fecal swabs from abattoirs' workers were randomly collected from different abattoirs located at Dakahlia governorate, Egypt. The Prevalence rate of L. monocytogenes in cattle carcasses was 3 (12%), 2 (8%) and 1(4%) in examined liver, kidneys and heart samples out of 25 examined samples for each organ, respectively and couldn't be detected in any of muscle samples. Meanwhile, in buffalo carcasses, theprevalence rate was 4 (16%), 2 (8%),1 (4%) and 1 (4%) in examined liver, kidneys, heart and muscle samples out of 25 examined samples for each organ, respectively. Meanwhile, in sheep carcasses, the prevalence rate of L. monocytogenes was 3 (12%), 2 (8%), 2 (8%) and 2 (8%) in examined liver, kidneys, heart and muscle samples out of 25 examined samples for each organ, respectively. Meanwhile, in goat carcasses, the prevalence rate was 4 (12%), 2 (8%), 2 (8%) and 1(4%) in examined liver, kidneys, heart and muscle samples out of 25 examined samples for each organ, respectively. In environmental samples, the prevalence rate was 28% from examined wall swabs before cleaning, 4% from examined wall swabs after cleaning, 4% from examined knife swabs and 4% from fecal swabs from abattoirs workers. Meanwhile, no L. monocytogenes was detected in water samples. Regarding pathogenicity test, death of all inoculated wining rabbits (23) as following 60% on 3rd day, 30% on 4th day and 10% on 5th day and L. monocytogenes was isolated from their organs. The isolates of L. monocytogenes were found to be virulent by using PCR assay incorporating inlA, hlyA and prfA genes primers. The public health hazards as well as suggestive measures to reduce human listeriosis have been discussed.

Highlights

IntroductionListeriosis in human poses a risk to pregnant women, newborn infants (called prenatal Listeriosis and represent one third of human listeriosis) and immunocompromised individuals (called adult Listeriosis and represent two third of human Listeriosis) and has a high mortality rate in these individuals of 20% to 30% (Gracieux et al, 2003 and Rocourt et al, 2000), healthy individuals could develop milder form of gastrointestinal illness (Dalton et al, 1997)
Listeriosis in human poses a risk to pregnant women, newborn infants and immunocompromised individuals and has a high mortality rate in these individuals of 20% to 30% (Gracieux et al, 2003 and Rocourt et al, 2000), healthy individuals could develop milder form of gastrointestinal illness (Dalton et al, 1997)
(+ve control) 3rd day 4th day 5th day. It is evident from the results achieved in Table (2) that liver samples had the highest prevalence of L. monocytogenes 3 (12%), followed by kidney samples 2 (8%), and heart samples 1(4%) and no L. monocytogenes were detected in muscle samples

Summary

Introduction

Listeriosis in human poses a risk to pregnant women, newborn infants (called prenatal Listeriosis and represent one third of human listeriosis) and immunocompromised individuals (called adult Listeriosis and represent two third of human Listeriosis) and has a high mortality rate in these individuals of 20% to 30% (Gracieux et al, 2003 and Rocourt et al, 2000), healthy individuals could develop milder form of gastrointestinal illness (Dalton et al, 1997). Death is rare in healthy adults but can occur at a rate as high as 30% in persons at highest risk (Demetios et al, 1996). L. monocytogenes can enter through infected animals and raw meat or intermediate products processed by suppliers (Nesbakken et al, 1996 and Sammarco et al, 1997). Animals are considered to be a part of food chain as important producers of meat and milk which provide high quality proteins and a key role in supplying calories (ESAP, 2001)

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