Abstract
Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, we concluded that they belong to a single LRV2 species. New viruses are closely related to the LRV2-Lmj-ASKH documented in Turkmenistan in 1995, which is congruent with their shared host (L. major) and common geographical origin.
Highlights
IntroductionThe family includes similar viruses found in other protists (Giardia, Trichomonas, Eimeria) and various fungi [1]
Leishmaniavirus is a genus of the family Totiviridae
We present sequence and phylogenetic analysis of two newly identified leishmania this paper, we present sequence and phylogenetic ofin two newly identified leishmania virusesIn (LRV2)
Summary
The family includes similar viruses found in other protists (Giardia, Trichomonas, Eimeria) and various fungi [1]. Their virions are icosahedral (T = pseudo 2), non-enveloped, and approximately 40 nm in diameter [2,3,4]. The doublestranded RNA (dsRNA) genome of Leishmaniavirus is not segmented, is approximately 5.2 kb in length, and contains two open reading frames (ORFs) for the capsid protein and the RNA-dependent RNA polymerase (RDRP) [5,6]. The mechanism of fusion Gag-Pol protein production varies, with RDRP located in +1 or -1 frameshift relative to or in-frame with the capsid [7,8]
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