Abstract
The plant pathogenic leaf spot fungi cause loss in crop yield. Fungi and other pathogens such as a virus, bacteria, cause leaf spot diseases and nematodes play a secondary role. Therefore, it is of interest to study internal transcribed spacer (ITS) sequence from the plant pathogenic fungi. Hence, we collected nineteen different isolates at Madurai Kamaraj University, Tamilnadu, India for this study. We report nineteen positive isolates identified with species-level characterization using ITS sequence supported with a phylogenetic tree and corresponding secondary structure analysis.
Highlights
Plants are an excellent source for the innovation of new products with medicinal importance in drug development
Overall 19 leaf spot fungi were isolated based on the culture morphology, color and mycelial growth patterns. 19 isolates were differentiated into six genera such as Colletotrichum sp., Diaporthe/Phomopsis sp., Guignardia sp., Phoma sp., Nigrospora sp., Alternaria sp, (Table 1)
Based on the polymerase chain reaction (PCR) analysis the range of 500 to the 600bp length of internal transcribed spacer (ITS) regions was observed in 1% Agarose Gel Electrophoresis
Summary
Plants are an excellent source for the innovation of new products with medicinal importance in drug development. The secondary metabolites are economically very important as drugs, fragrances, pigments, flavors, food sources, and pesticides. All the Fungi are rich sources of thousands of secondary metabolites. A few reports available about Leaf spot fungi and their identification at the species level. The ITS region is composed of ITS1/ITS2 intergenic sequences with well conserved 5.8 rRNA in between. ITS2 sequence variability is thought to be suitable to differentiate species and for phylogenetic reconstructions, which can be further enhanced if structural information is considered. The ITS region is the universally sequenced genetic marker for fungal identification. The ITS has commonly used the sequence for many studies and fourteen thousand fully known fungal species available in the public sequence databases
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