Abstract
BackgroundKlebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen associated with community-acquired infections and nosocomial infections. From 2010 to 2015, K. pneumoniae testing was included into the exiting diarrhea-syndrome surveillance with objective to estimate the prevalence of K. pneumoniae in diarrhea-syndrome patients, test antibiotics susceptibility and investigate molecular characteristics.MethodsStool specimens from diarrhea-syndrome outpatients were cultured and identified the pathogens by the Vitek2 Compact instrument. The isolated K. pneumoniae strains were tested for antibiotics susceptibility by minimal inhibitory concentration (MIC) method, and subtyped by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).Results22 K. pneumoniae strains were identified from 4340 stool specimens of outpatients who visited sentinel hospitals in Beijing during 2010–2015. All strains were sensitive to gentamicin, nalidixic acid, ciprofloxacin, ceftriaxone, cefotaxime, cefepime, imipenem. The highest resistance rate of K. pneumoniae strains was 100% to amoxicillin–clavulanate, followed by 72.7% to ampicillin. These 22 K. pneumoniae strains were characterized into 21 different PFGE types and 20 MLST types with less similarity.ConclusionsThe detection rate of K. pneumoniae in stool specimens from outpatients with diarrhea syndromes was about 0.5% in Beijing. Less similarity of the isolated strains indicated the unlikely long-term circulating of K. pneumoniae in the community. ST23 was the most common genotype. Drug resistance of the community-acquired K. pneumoniae was not a serious problem in comparing with hospital-acquired infections. High vigilance in the community-acquired K. pneumoniae strains and investigation of pathogens’ microbiological characteristics are valuable for signals detection for drug resistance in population and strains variation.
Highlights
Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen associated with communityacquired infections and nosocomial infections
Identification of bacterial strains In the 4340 stool specimens collected in the surveillance period from 2010 to 2015, 22 K. pneumoniae strains were identified: one strain in 2010, three strains in 2011 and 2012 respectively, five strains in 2013, nine strains in 2014, and one strain in 2015; 16 out of 22 positive K. pneumoniae were isolated from April to October, while six positives identified in low-incidence seasons 2010–2015
All 22 K. pneumoniae strains were sensitive to ceftriaxone, cefotaxime, the minimal inhibitory concentration (MIC) was less than 1 μg/mL, and no extended-spectrum beta-lactamases (ESBL) production strain was identified
Summary
Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen associated with communityacquired infections and nosocomial infections. From 2010 to 2015, K. pneumoniae testing was included into the exiting diarrhea-syndrome surveillance with objective to estimate the prevalence of K. pneumoniae in diarrhea-syndrome patients, test antibiotics susceptibility and investigate molecular characteristics. Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen associated with both community-acquired and nosocomial. Stool specimens were collected from diarrhea-syndrome outpatients in sentinel hospitals [4], and tested the common diarrhea induced pathogens including rotavirus, norovirus, diarrheagenic Escherichia coli, Salmonella, and Shigella spp. Lu et al Gut Pathog (2017) 9:39 increasingly nosocomial infections caused by K. pneumoniae might impose an increasing risk of infections in communities, from 2010 to 2015, K. pneumoniae testing was included into the exiting diarrhea-syndrome surveillance with objective to estimate the prevalence of K. pneumoniae in diarrheasyndrome outpatients, test antibiotics susceptibility and investigate microbiological characteristics
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