Molecular characterization of jasmine genotypes using RAPD and ISSR markers

Abstract

Understanding the genetic background of existing germplasm represents a value-added component of managing collections. Molecular approaches collectively represent a potential tool for gathering information on genetic improvement. Keeping in view, the present investigation was carried out to explore the molecular diversity among 18 jasmine genotypes by employing two DNA based molecular marker techniques, viz., random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR). A total of 33 polymorphic primers (20 RAPD and 13 ISSR) were used. Amplification of genomic DNA of 18 genotypes, using RAPD analysis resulted in a total of 248 products, out of which 234 (94.35%) products were polymorphic. The polymorphism percentage ranged from 80–100% with an average of 94.24% polymorphism per primer. In ISSR analysis, among thirteen primers tested, a total of 595 products were produced out of which 562 (94.45%) products were polymorphic. The polymorphism ranged from 82.60–100% per cent, with an average of 94.45% polymorphism per primer. Based on the similarity matrix data, dendrograms were generated using UPGMA method. The genotypes were categorized into several groups and subgroups which varied distinctly with J. primulinum forming a separate cluster.