Abstract
Fusarium is a diverse and heterogeneous fungi genus. Its wide genetic variability and similarity in morphological characteristics hinder the identification of species of this genus. Identifying Fusarium species is difficult due to the genus. Several molecular methods have been useful for differentiating these species, and the amplification of internal transcribed spacer (ITS) regions of the fungus ribosomal DNA has been successfully used, since ITS are preserved regions of the DNA that assists in distinguishing species. The objective of this work was to collect and characterize isolates of Fusarium spp. associated with wilt symptoms in Capsicum spp. in the biomes of the state of Mato Grosso, Brazil. Were collected tissue samples of plants with wilt symptoms. The DNAs of the Fusarium spp. found were extracted, and subjected to polymerase chain reaction, using the primers ITS1 (5’TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’). Subsequently, the sequencing was performed. The resulting sequences were, five Fusarium species were found F. solani, F. oxysporum, F. equiseti, F. incarnatum, F. chlamydosporum, predominating F. solani and F. equiseti.
Highlights
Fusarium is a diverse and heterogeneous fungi genus that is important in the food and drug industry, medicine, and agriculture (Luginbuhl, 2010)
Several pathogens cause wilt in Capsicum spp. (Ochoa & Ramirez, 2001; Naik et al, 2008; Singh et al, 2017), wilt caused by Fusarium, especially Fusarium solani and Fusarium oxysporum, is the main disease in peppers and chilies, and a serious problem for these crops in the last years (Raghu et al, 2016; Tembhurne et al, 2017)
Eighty-nine plants of Capsicum spp., collected in the biomes of the state of Mato Grosso, presented characteristic symptoms of those caused by Fusarium—wilt, yellowing, tipping, and stem base necrosis
Summary
Fusarium is a diverse and heterogeneous fungi genus that is important in the food and drug industry, medicine, and agriculture (Luginbuhl, 2010). Fusarium species are worldwide spread; this species have greater genetic, and pathogenic variability (Gonçalves, 2015). They are known for their ability to cause several diseases in diverse hosts (Summerell et al, 2003). One of the predominant characteristics of Fusarium species is the formation of asexual spores—macroconidia and microconidia (Teixeira, 2015) They produce resistance structures called chlamydospores (Bedendo, 1995), which assures their survival in plant and soil debris for many years and makes their control and eradication difficult (Raghu et al, 2016; El Kichaoui et al, 2017)
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