Molecular characterization of integrons and Genotyping of Salmonella enterica isolated from diarrheal animals and humans

Abstract

Resistance to antibacterial drugs is a major health problem because of its impact . It is important to study the prevalence of antibiotic resistance genes in order to understand.Twenty five Salmonella enterica isolated from human and animal diarrhea. Traditional culture methods, biochemical methods, and molecular methods were used to characterize the samples during December to September 2020 to 2021. Disk diffusion method was used to determine antimicrobial susceptibility patterns of isolates identified as MDR by the Clinical and Laboratory Standards Institute (CLSI). The PCR amplification was used to show presence of class 1, 2 and 3 integrons. Class 1 integrons and class3 were detected in 100% of the multidrug-resistant Salmonella isolates,whlie class2 detected among 40%. RAPD-PCR fingerprinting regarded successfully as a reliable, reproducible, accurate and sensitive discriminatory method to Salmonella enterica. The results of RAPD-PCR amplification of Salmonella enterica isolates revealed that 24 isolates (96.%) also showed about one to six amplification bands per isolate, except one isolates were untypeble. The most frequent band was a 1400bp band (72%), while the lowest frequent band was 400bp (8%) among an individual isolates.