Molecular characterization of immunoglobulin (Ig) genes reveals that plasmablasts generated during heterologous secondary dengue infections and circulating memory B cells after recovery are of distinct origin

Abstract

Event Abstract Back to Event Molecular characterization of immunoglobulin (Ig) genes reveals that plasmablasts generated during heterologous secondary dengue infections and circulating memory B cells after recovery are of distinct origin Ramapraba Appanna1, Xu Mei Hui1, Toh Ying Xiu1, Thavamalar Balakrishnan1, Srinivasan Kandhadayar Gopalan1, Francesca Zolezzi1, Michael Poidinger1, Leo Yee Sin2, Wang Cheng-I1 and Katja Fink1* 1 Singapore Immunology Network, Singapore 2 Tan Tock Seng Hospital, Department of Infectious Diseases, Singapore Memory B cells generated after exposure to dengue viral (DENV) infection are a central component in shaping immune memory. However, re-exposure to dengue virus of a different serotype is often associated with an increased disease pathogenesis. During repeated infection, the rapid activation of memory B cells leads to the generation of massive amounts of cross-reactive antibodies. In the acute phase of a secondary infection, this B cell memory pool probably outcompetes the newly generated virus specific B cells. To characterize the specificity of plasmablasts and memory B cells generated during and after acute infection, we performed single cell RT-PCR for sequence analysis of Ig variable regions. Surprisingly, we found that memory B cells isolated one month after disease were of different genetic composition than acute phase plasmablasts. Our data reveal preferential usage of the Ig heavy chain variable region gene VH1 in DENV binding plasmablasts. In contrast, VH3 family repertoires were noted in specific long lasting circulating memory cells isolated over a month after infection. The antibodies isolated from these memory B cells had a broad reactivity, with only 30% being specific to DENV envelope (E) protein, whereas 70-80% plasmablasts were specific to E protein. Whether VH gene usage differs in primary and secondary infected patients and reflects a specific antigen/epitope selection is currently under investigation. This study can help to understand immune mechanism of protection for potential application in vaccination. Keywords: Dengue, memory B cell, plasmablast, immunoglobulin, repertoire Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Appanna R, Mei Hui X, Ying Xiu T, Balakrishnan T, Kandhadayar Gopalan S, Zolezzi F, Poidinger M, Yee Sin L, Cheng-I W and Fink K (2013). Molecular characterization of immunoglobulin (Ig) genes reveals that plasmablasts generated during heterologous secondary dengue infections and circulating memory B cells after recovery are of distinct origin. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00905 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Katja Fink, Singapore Immunology Network, SINGAPORE, Singapore, katjafink3@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ramapraba Appanna Xu Mei Hui Toh Ying Xiu Thavamalar Balakrishnan Srinivasan Kandhadayar Gopalan Francesca Zolezzi Michael Poidinger Leo Yee Sin Wang Cheng-I Katja Fink Google Ramapraba Appanna Xu Mei Hui Toh Ying Xiu Thavamalar Balakrishnan Srinivasan Kandhadayar Gopalan Francesca Zolezzi Michael Poidinger Leo Yee Sin Wang Cheng-I Katja Fink Google Scholar Ramapraba Appanna Xu Mei Hui Toh Ying Xiu Thavamalar Balakrishnan Srinivasan Kandhadayar Gopalan Francesca Zolezzi Michael Poidinger Leo Yee Sin Wang Cheng-I Katja Fink PubMed Ramapraba Appanna Xu Mei Hui Toh Ying Xiu Thavamalar Balakrishnan Srinivasan Kandhadayar Gopalan Francesca Zolezzi Michael Poidinger Leo Yee Sin Wang Cheng-I Katja Fink Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.