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Abstract
Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite. Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819. Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.
Highlights
Hymenolepis nana is a zoonotic tapeworm with widespread distribution
polymerase chain reaction (PCR)-sequencing analysis of ribosomal internal transcribed spacer 2 (ITS2) fragment was targeted to investigate the molecular identification of the parasite
Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration
Summary
Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. Hymenolepis nana, generally known as the dwarf tapeworm, is one of the most common tapeworms of humans and rodents, in which the parasite can cause hymenolepiasis. This zoonotic tapeworm has a cosmopolitan distribution with socio-economic and medical significance which may occur in many countries, worldwide[1,2]. With advancement in the field of molecular biology, techniques such as polymerase chain reaction (PCR) has provided simple and rapid procedure in identification of parasites This approach is a widely used method for the accurate differentiation and characterization of helminthic parasites including the cestodes[14,17]. The main objective of the present study was to molecular characterization of H. nana in Qazvin province, North West of Iran
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